Winterhardiness is a basic trait for successful winter survival barley (Hordeum vulgare L.) crop. Freezing tolerance, a fundamental component of winterhardiness, is based on an inducible process known as hardening or cold acclimation that occurs when plants are exposed to low non-freezing temperatures. In the recent years, many temperature-dependent genes speciﬁcally expressed during hardening have been isolated. Current data on relationship between gene expression and cold tolerance are mostly based on plants grown and hardened under environmentallycontrolledconditionsand, usually, overa shortperiodof time. Inorderto verifywhethervariations in the molecular response to cold are likely to be of signiﬁcant adaptive value under natural environments, we have followed the accumulation of several COR genes (pt59, pao86 and paf93) and proteins (COR14a and COR14b) during the 1996/97 and 97/98 winter seasons in barley cultivars with contrasting winterhardiness capacity grown under ﬁeld conditions. In the 1996/97 experiment, a winter cultivar Onice and a spring cultivar Gitane were tested for the accumulation of the cold-regulated genes and proteins. The ability of the plants to promote a strong molecular response to cold was found to be associated with the winterhardiness capacity of the two cultivars. This result was further tested in the winter season 1997/98 using 10 barley varieties. All winter cultivars showed high accumulation of the cold-regulated proteins COR14a and COR14b, while some variations for this character were detected in the spring cultivars suggesting that the selection for winter survival has been effective to ﬁx the high COR14 accumulation capacity. We conclude that a high level of COR14 may be a component the winter survival capacity of barley. 1999 Kluwer Academic Publishers. Printed in the Netherlands.
|Autori:||Giorni, E.;Crosatti, C.;Baldi, P.;Grossi, M.;Marè, C.;Stanca, A.M.;Cattivelli, L.|
|Data di pubblicazione:||1999|
|Titolo:||Cold-regulated gene expression during winter in frost tolerant and frost susceptible barley cultivars grown under field conditions|
|Appare nelle tipologie:||1.1 Articolo in rivista|