Oleuropein (OL) and hydroxytyrosol (HT), the main olive oil polyphenols, possess anti-proliferative effects in vitro. Fatty acid synthase, a key anabolic enzyme of biosynthesis of fatty acids, plays an important role in colon carcinoma development. Our aim was to investigate whether gene expression of FAS, as well as its enzymatic activity, is regulated by HT and OL in two human colon cancer cell lines, as HT-29 and SW620. In addition, we investigated the effects of these polyphenols on growth and apoptosis in these cells. FAS gene expression and activity in treated HT-29 and SW620 cells were evaluated by realtime PCR and radiochemical assay, respectively. Cell growth and apoptosis, after polyphenols treatment, were measured by MTT test and flow cytometry, respectively. The inhibition of proliferation, detected after HT treatment, was mediated by an inhibition of FAS expression and its enzymatic activity in SW620 cells, while the anti-proliferative effect in HT-29 cells seems to be independent from FAS. OL exerted an anti-proliferative effect only on SW620 cells with a mechanism which excluded FAS.
|Autori:||Notarnicola, M.;Pisanti, S.;Tutino, V.;Bocale, D.;Rotelli, M.T.;Gentile A.;Memeo, V.;Bifulco M.;Perri, E.;Caruso, M.G.|
|Data di pubblicazione:||2011|
|Titolo:||Effects of olive oil polyphenols on fatty acid synthase gene expression and activity in human colorectal cancer cells|
|Rivista:||GENES AND NUTRITION|
|Appare nelle tipologie:||1.1 Articolo in rivista|