Leptin is an adipocyte-derived hormone which acts as a major regulator for food intake and energy homeostasis. Previous studies have focused on the association between polymorphisms in the coding region of the leptin (LEP) pig gene and economically important traits. The present work aimed to study the leptin gene at the promoter region to search for polymorphisms that could lead to different gene expression regulation. In the 1213 bp 5’ gene flanking region, we identified 17 new polymorphisms in two pig breeds characterised by different fat content, namely Casertana (CT) and Large White (LW). In order to investigate whether a polymorphism affects the transcriptional activity of the leptin promoter, we evaluated in vitro reporter gene activity driven by two constructs harbouring different naturally occurring haplotypes, and we found a two-fold difference in transcription efficiency (P<0.04). These findings may serve as a basis for future studies even in different breeds and might provide molecular data that can be used in association with phenotypic data and finally in the selection scheme.
|Autori:||Crisà, A.;D’Andrea, M.;Willems, D.;Pilla, F.;Valentini, A.|
|Data di pubblicazione:||2011|
|Titolo:||SNPs identification in swine leptin 5’ flanking region and transcriptional activity of naturally occurring promoter haplotypes|
|Rivista:||ITALIAN JOURNAL OF ANIMAL SCIENCE|
|Appare nelle tipologie:||1.1 Articolo in rivista|