In this research, soil microbial structures under a wheat triennial monoculture and horse bean- wheat-horse bean succession were evidenced using a metagenomic approach. Polymorphism analysis of DNA extracted from soil samples collected at the end of the third year of the two crop successions, was performed by PCR, car- ried-out with six different primers designed on simple tandem repeats sequences. Readable pro- files were obtained with M13 primer, from which no polymorphisms were detected, and with the primer (GACA)4, that gave distinctive patterns. Experimental findings suggest that metage-nomic analysis performed by (GACA)4 primer may be an easy and suitable method to dis-criminate microbial diversity of different crop successions. (GACA)4 PCR-pattern indicate that soil microbiota changes are well correlated with crop succession.
|Autori:||Zaccardelli, M.;Villecco, D.;Campanile, F.;Pane, C.|
|Data di pubblicazione:||2012|
|Titolo:||Metagenomic profiles of soil microbiota under two different cropping systems detected by STRs-based PCR|
|Appare nelle tipologie:||1.1 Articolo in rivista|