Yellow colour is an important requirement for pasta production and breeding programs tend to select durum wheat varieties with high grain yellow pigment content (YPC). The aim of the present study was to detect quantitative trait loci (QTL) for YPC, yellow index (YI) and individual carotenoid compounds in a segregant population of 121 F2:F3-4 families, derived by crossing the cultivars Latino and Primadur, grown at four environments. Total carotenoids amounted to 37% of YP, indicating unknown colour-producing compounds in the durum extracts. Lutein was the most abundant carotenoid, followed by zeaxanthin, -carotene and -carotene, while -cryptoxanthin was minor component. Phytoene synthase marker Psy-A1, 150 SSR and EST-SSR markers, and 345 DArT® markers, were used to construct the linkage map for subsequent QTL analysis. Composite interval mapping identified five QTL on chromosomes 2A, 3B, 5A and 7A, accounting for a large proportion of the phenotypic variation for YPC and YI. Two major QTL for YPC were detected in two separate intervals on 7AL, thus confirming that allelic differences at Psy1, and at least one additional gene in the distal region of homoeologous group 7L are associated with differences in YPC. Clusters of QTL for total and/or one or more carotenoid compounds were detected on the same chromosome regions where QTL for YPC and YI were identified. The location and effect of QTL are discussed in relation to marker-assisted selection programs to facilitate high carotenoid concentration with high nutritional carotenoid compounds in durum wheat grain.
|Autori:||Blanco, A.;Colasuonno, P.;Schiavulli, A.;Gadaleta, A.;Mangini, G.;Simeone, R.;Digesù, A.M.;De Vita, P.;Mastrangelo, A.M.;Cattivelli, L.|
|Data di pubblicazione:||2011|
|Titolo:||Quantitative trait loci for yellow pigment content and individual carotenoid compounds in durum wheat|
|Rivista:||JOURNAL OF CEREAL SCIENCE|
|Appare nelle tipologie:||1.1 Articolo in rivista|