Abstract The genetic basis of stem celi specification in somatic embryogenesis and organogenesis is stili obscure. SOMA TIC EMBRYOGENESIS RECEPTOR-L/KE KINASE (SERK) genes are involved in embryogenesis and organogenesis in numerous species. In vitro culture of Cyclamen persicum immature ovules provides a system for investigating stem celi formation and maintenance, because lines forming either organs or embryos or callus without organs/embryos are available for the same cultivar and plant growth regulator conditions. The present aim was to exploit this property of cyclamen cultures to understand the role of SERK(s) in stem celi formation and maintenance in somatic embryogenesis and organogenesis in vitro, in comparison with expression in pianta. CpSERK1 and CpSERK2 were isolated from embryogenic callus. CpSERK1 and CpSERK2 levels by RT -PCR showed that expression is high in embryogenic, moderate in organogenic, and null in recalcitrant calli. in situ hybridizations showed that the expression of both genes started in clumps of pluripotent stem cells, from which both pre-embryogenic aggregates and organ meristemoids derived, and continued in their trans-amplifying, meristem-like, derivatives. Expre5sion declined in organ meristemoids, in parallel with a partial 105S of meristematization. In mature somatic embryos, and in shoot and root primordia, CpSERK1 and CpSERK2 were expressed in meristems, and similar patterns occurred in zygotic embryo and primary meristems in pIanta. The results point to SERK1 and SERK2 as markers of pluripotency in cyclamen. It is proposed that the high expression of these genes in the trans-amplifying derivatives of the stem cells maintains a pluripotent condition leading to totipotency and, consequently, somatic embryogenesis.
|Autori:||Savona, M.;Mattioli, R.;Nigro, S.;Falasca, G.;Della Rovere, F.;Costantino, P.;De Vries, S.;Ruffoni, B.;Trovato, M.;Altamura, M.M.|
|Data di pubblicazione:||2012|
|Titolo:||Two SERK genes are markers of pluripotency in Cyclamen persicum Mill.|
|Rivista:||JOURNAL OF EXPERIMENTAL BOTANY|
|Appare nelle tipologie:||1.1 Articolo in rivista|