SLC11A1 (natural-resistance-associated macrophage protein) gene influences the initial phase of bacterial cellular infections, regulating macrophage activation. Recent literature on buffalo has attempted to associate the genotypes at the polymorphic microsatellite, that is located in the 3’untraslated region (3’UTR) of the gene, with either susceptibility to brucellosis or with improved macrophage function. Some authors reported the (GT)16 carrier as resistant animals to brucellosis (Capparelli et al., 2007a). In this paper we aimed to analyse the steady-state level of SLC11A1 expression in a serologically negative herd on 26 animal differing at the (GT)n microsatellite repeats by using the reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR). To perform this study we evaluated five different reference gene (RG) not reported previously to be used in buffalo blood gene expression experiments. Finally we did not find statistically significant difference between buffaloes carriers of different alleles at the 3’UTR, in NRAMP1 expression either in whole blood , or in the blood fractions (peripheral blood mononuclear cells (PBMC) and polymorphonuclear leukocytes/granulocytes (PMN/G). On the contrary, significant difference was found in the SLC11A1 expression between the different blood fractions, with PMN/G fraction having a higher expression level compared to PBMC (P<0.015).
|Autori:||Crisà, A.;De Matteis, G.;Scatà, M.C.;Moioli, B|
|Data di pubblicazione:||2013|
|Titolo:||Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells by using qPCR|
|Rivista:||GENETICS AND MOLECULAR RESEARCH|
|Appare nelle tipologie:||1.1 Articolo in rivista|