Background: Durum wheat often faces water scarcity and high temperat ures, two events that usually occur simultaneously in the fields. Here we report on the stress responsive strategy of two durum wheat cultivars, characterized by different water use efficiency, subjected to drought, heat and a combination of both stresses. Results: The cv Ofanto (lower water use efficiency ) activated a large set of well-known drought-related genes after drought treatment, while Cappelli (higher water use efficiency) showed the constitutive express ion of several genes induced by drought in Ofanto and a modulation of a limited number of genes in response to stress. At molecular level th e two cvs differed for the activation of molecular messengers, genes involved in the regulation of chromatin condensation, nuclear speckles and stomat al closure. Noteworthy, the heat response in Cappelli involved also the up-regulation of genes belonging to fatty acid β -oxidation pathway, glyoxylate cycle and senescence, suggesting an early activation of senescence in this cv. A gene of unknown function having the greatest expression difference between the two cultivars was selected and used for express ion QTL analysis, the corresponding QTL was mapped on chromosome 6B. Conclusion: Ofanto and Cappelli are characterized by two opposite stress-responsive strategies. In Ofanto the combinati on of drought and heat stress led to an increased number of modulated genes, exceeding the simple cumulative effects of the two singles tresses, whereas in Cappelli the same treatment triggered a number of differentially expressed genes lower than t hose altered in response to heat stress alone.This work provides clear evidences that the gene tic system based on Cappelli and Ofanto represents an ideal tool for the genetic dissection of the molecular response to drought and other abiotic stresses.
|Autori:||Aprile, A.;Havlickova, L.;Panna, R.;Marè, C.;Borrelli, G.M.;Marone, D.;Perrotta, C.;Rampino, P.;De Bellis, L.;Curn, V.;Mastrangelo, A.M.;Rizza, F.;Cattivelli, L.|
|Data di pubblicazione:||2013|
|Titolo:||Different stress responsive strategies to drought and heat in two durum wheat cultivars with contrasting water use efficiency|
|Appare nelle tipologie:||1.1 Articolo in rivista|