Ladino white clover evolved in the Lombardy plain and has largely been used for cultivation and breeding. Different authors hypothesized its origin from Dutch clover (domesticated in the 16th Century) rather than indigenous wild populations. This study aimed to provide insight into the origin of Ladino, as well as assessing the consistency between morphophysiological and SSR marker information and the extent of among-population and within-population genetic variation in white clover. One landrace and one wild population of Ladino, four Italian non-Ladino wild populations collected along a latitudinal gradient at different elevations, and one Fries-Groninger (Dutch) landrace, were evaluated for 10 morphophysiological traits. Twenty-five individuals per population were genotyped by 32 well-distributed SSR markers. Population ordination and classification of molecular diversity based on Nei’s  or Reynolds’ distance, and structure analysis, did not support the origin of Ladino from Dutch germplasm. Molecular marker-based ordination of the Italian wild populations reflected the geographical and altitudinal similarity of their collecting sites. Within-population molecular variation did not differ among populations, and was about ten-fold greater than among-population variation. Morphophysiological diversity of the populations was substantially unrelated to molecular diversity, while reflecting evolutionary adaptation to environments of origin.
|Autori:||Annicchiarico, P.;Carelli, M.|
|Data di pubblicazione:||2014|
|Titolo:||Origin of Ladino white clover as inferred from patterns of molecular and morphophysiological diversity|
|Appare nelle tipologie:||1.1 Articolo in rivista|