Accessions of Kolkwitzia amabilis Graebn. of wild origin with collection information and cultivated sourceswith or without collection information were grown under the same environments to observe the dif-ferences in morphology and flowering time. The morphological variation was further documented byanalyzing the nuclear internal transcribed spacers 1, 2 in ribosomal RNA gene (ITS 1, 2 region) and ran-domly amplified polymorphic DNA (RAPD) markers. US National Arboretum accession NA64889 of wildorigin with collection information (known wild origin) flowered earlier than cultivated sources with(cultivars of known wild origin) or without (cultivars of unknown wild origin) collection information fortwo years. However, significant variations were observed in sequences of the polymerase chain reaction(PCR) products of all clones of all accessions with the exception of AA20447C. Four out of five clones ofNA64889 showing haplotype H were together in the same sub-clusters separated from other accessions.We conclude that NA64889 of known wild origin that flowers early was different from other accessionsof cultivated sources of known wild origin (AA816-84A) or of unknown wild origin (AA20447 and AA181-2005). Moreover, the analysis performed on the on the ITS 1, 2 region sequence and RAPD markers wereshown that all accessions of cultivated origin, analyzed in this study, are derived from germplasm ofknown wild origin (AA816-84A).
|Autori:||Park, S.H.;Burchi, G.;Roh, M.S.;Joung Y.H.|
|Data di pubblicazione:||2014|
|Titolo:||Characterization of Kolkwitzia amabilis accessions based on flowering and molecular markers|
|Appare nelle tipologie:||1.1 Articolo in rivista|