The phytopathogen Pseudomonas syringae pv. actinidiae (Psa) is the causal agent of bacterial canker of kiwifruit. In the last years, it has caused severe economic losses to Actinidia spp. cultivations, mainly in Italy and New Zealand. Conventional strategies adopted did not provide adequate control of infection. Phage therapy may be a realistic and safe answer to the urgent need for novel antibacterial agents aiming to control this bacterial pathogen. In this study, we described the isolation and characterization of two bacteriophages able to specifically infect Psa. ϕPSA1, a member of the Siphoviridae family, is a temperate phage with a narrow host range, a long latency, and a burst size of 178; ϕPSA2 is a lytic phage of Podoviridae family with a broader host range, a short latency, a burst size of 92 and a higher bactericidal activity as determined by the TOD value. The genomic sequence of ϕPSA1 has a length of 51,090bp and a low sequence homology with the other siphophages, whereas ϕPSA2 has a length of 40472bp with a 98% homology with Pseudomonas putida bacteriophage gh-1. Of the two phages examined, ϕPSA2 may be considered as a candidate for phage therapy of kiwifruit disease, while ϕPSA1 seems specific toward the recent outbreak's isolates and could be useful for Psa typing.
|Autori:||Di Lallo, G.;Evangelisti, M.;Mancuso, F.;Ferrante, P.;Marcelletti, S.;Tinari, A.;Superti, F.;Migliore, L.;D’Addabbo, P.;Frezza, D.;Scortichini, M.;Thaller, M.C.|
|Data di pubblicazione:||2014|
|Titolo:||Isolation and partial characterization of bacteriophages infecting Pseudomonas syringae pv. actinidiae, causal agent of kiwifruit bacterial canker|
|Rivista:||JOURNAL OF BASIC MICROBIOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|