A survey of grapevine viruses’ presentce in the region of Calabria (southern Italy) was carried out, and the sanitary selection was conducted on various autochthonous indigenous varieties. Serological (ELISA) and molecular (Multiplex RT-PCR) tests were used to detect the viruses included in the Italian certification programme: Arabis mosaic virus (ArMV), Grapevine fanleaf virus (GFLV), Grapevine leafroll associated virus 1 (GLRaV-1), Grapevine leafroll associated virus 2 (GLRaV-2), Grapevine leafroll associated virus 3 (GLRaV-3), Grapevine virus A (GVA), Grapevine virus B (GVB), Grapevine fleck virus (GFkV). The frequency with which the above viruses have been detected was 37.4%, 32.6%, 12.8%, 7.7%, 7.3%, 1.9% e 0.3% respectively for GVA, GLRaV-3, GFLV, GFKV, GLRaV-1, GLRaV-2 and GVB.; ArMV was never found. This sanitary selection allowed for the detection of 6 putative clones of ‘Arvino’, 2 of ‘Magliocco dolce’ and 2 of the rootstock ‘17-37’ free of the above-mentioned viruses. The necessary process for the commercialisation of these clones as “certified” propagation material was accomplished, and their official approval by the Italian Ministry of Agriculture is currently in progress.
|Autori:||Leo G.;Luison D.;Grande S.;Albanese G.;Faggioli F.|
|Data di pubblicazione:||2015|
|Titolo:||GRAPEVINE VIRUSES’ DETECTION AND SANITARY SELECTION IN GRAPEVINE GERMPLASM OF CALABRIA (SOUTHERN ITALY)|
|Rivista:||JOURNAL OF PHYTOPATHOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|