Diversity array technology (DArT) markers are largely used for mapping, genetic diversity, and association mapping studies. For years, they have been used as anon- ymous genomic markers, as their sequences were not known. As the sequences of 2,000 wheat DArT clones are now available, this study was designed to analyze these sequences with bioinformatic approaches, and to study the genetic features of a subset of 291 markers positioned on the A and B genomes in three durum wheat genetic maps. A set of 1,757 non-redundant sequences was identiﬁed, and used as queries for similarity searches. Analysis of the genetic positions of markers corresponding to nearly identical sequences indicates that redundancy of sequences is one of the factors that explains the clustering of these markers in speciﬁc genomic regions. Of a total of 1,124 DArT clones (64 %) that represent putatively expressed sequences, putative functions are proposed for more than 700 of them. Of note, many clones correspond to genes that are related to disease resistance, as characterized by leu- cine-rich repeat domains, and 40 of these clones are posi- tioned in the three genetic maps presented in this study. Finally, DArT markers have been used to ﬁnd syntenic regions in the Brachypodium and rice genomes. In con- clusion, the analyses herein presented contribute to explain the main features of DArT markers observed in genetic maps, as clustering in short chromosome regions. More- over, the attribution of putative gene functions for more than 700 sequences makes these markers an optimal tool for collinearity studies or for the identiﬁcation of candidate genes.
|Autori:||Marone, D.;Panio, G.;Ficco, D.B.M.;Russo, M.A.;De Vita, P.;Papa, R.;Rubiales, D.;Cattivelli, L.;Mastrangelo, A.M.|
|Data di pubblicazione:||2012|
|Titolo:||Characterization of wheat DArT markers: genetic and functional features|
|Rivista:||MOLECULAR GENETICS AND GENOMICS|
|Appare nelle tipologie:||1.1 Articolo in rivista|