The genus Gladiolus L. (Iridaceae) includes important ornamental species and hybrids which are successfully treated on the market since the last century. Recently breeders have selected new genotypes with the aim to increase the production in winter and late spring especially in countries bordering the Mediterranean Sea. In order to speed up the volume of the new hybrids and to produce virus-free stocks of mother plants, it is possible for Gladiolus to apply biotechnological tools of in vitro propagation through liquid culture or simple bioreactors such as temporary immersion system to reduce production costs and to enhance multiplication rate and cormel quality. Gladiolus micropropagation was reported first by Ziv et al. in 1970 and subsequently by several other authors exploring the performances of different species and varieties. Bulbs and corms of several species are commercially propagated in liquid culture in semi-automatic systems as temporary immersion; in Gladiolus, Ruffoni et al. presented in 2008 data about high efficiency micropropagation using temporary immersion compared with the culture on agar-solidified medium suggesting an efficient semi automatized protocol. The present paper takes into consideration the different ways for in vitro culture initiation and the efficiency of the meristem excision for the establishment of pathogen-free cultures including data coming from direct experiences and bibliography search. Moreover it will compare the different growth strategies (solid vs liquid micropropagation) evaluating finally the performances of the temporary immersion system.
|Autori:||Ruffoni, B.;Savona, M.;Barberini, S.|
|Data di pubblicazione:||2012|
|Titolo:||Biotechnological Support for the Development of New Gladiolus Hybrids|
|Rivista:||FLORICULTURE AND ORNAMENTAL BIOTECHNOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|