The fermented manure derivative known as Preparation 500 is traditionally used as a field spray in biodynamic agriculture for maintaining and increasing soil fertility. This work aimed at characterizing the product from a microbiological standpoint and at assaying its bioactive properties. The approach involved molecular taxonomical characterization of the culturable microbial community; ARISA fingerprints of the total bacteria and fungal communities; chemical elemental macronutrient analysis via a combustion analyzer; activity assays for six key enzymes; bioassays for bacterial quorum sensing and chitolipooligosaccharide production; and plant hormonelike activity. The material was found to harbor a bacterial community of 2.38 × 108 CFU/g dw dominated by Grampositives with minor instances of Actinobacteria and Gammaproteobacteria. ARISA showed a coherence of bacterial assemblages in different preparation lots of the same year in spite of geographic origin. Enzymatic activities showed elevated values of β-glucosidase, alkaline phosphatase, chitinase, and esterase. The preparation had no quorum sensing-detectable signal, and no rhizobial nod gene-inducing properties, but displayed a strong auxin-like effect on plants. Enzymatic analyses indicated a bioactive potential in the fertility and nutrient cycling contexts. The IAA activity and microbial degradation products qualify for a possible activity as soil biostimulants. Quantitative details and possible modes of action are discussed.
|Autori:||Giannattasio, M.;Vendramin, E.;Fornasier, F.;Alberghini, S.;Zanardo, M.;Stellin, F.;Concheri, G.;Stevanato, P.;Ertani, A.;Nardi, S.;Rizzi, V.;Piffanelli, P.;Spaccini, R.;Mazzei, P.;Piccolo, A.;Squartini, A.|
|Data di pubblicazione:||2013|
|Titolo:||Microbiological features and bioactivity of a fermented manure product (Preparation 500) used in biodynamic agriculture.|
|Rivista:||JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|