Fusarium oxysporum f. sp. melonis (FOM) is the cause of melon vascular wilting, the most severe infectious disease of this cucurbit. Four races (0, 1, 2, and 1,2) of FOM have been designated which are all present in Italy. A PCR assay based on race-specific SCAR markers for FOM race 2 identification was developed which proved specific, sensitive, and reliable regardless of the origin of isolates. This study demonstrated that SCAR primers developed from RAPD markers can be used to unambiguously identify FOM race 2 which causes similar symptoms as the other three FOM races which are morphologically indistinguishable to one another and to the other formae speciales of F. oxysporum. The specificity of these primers which did not amplify several formae speciales, and the most common melon pathogens, make them a reliable tool for diagnosis. This finding may have additional practical applications in the epidemiology and management of Fusarium wilt of melon caused by this race in areas where it is very widespread.
|Autori:||Luongo, L.; Vitale, S.; Haegi, A.; Belisario, A.|
|Data di pubblicazione:||2012|
|Titolo:||Development of scar markers and PCR assay for Fusarium oxysporum f. sp. melonis race 2-specific detection|
|Rivista:||JOURNAL OF PLANT PATHOLOGY|
|Appare nelle tipologie:||1.1 Articolo in rivista|