Apple scab resistance is one of the most well- characterized plant–pathogen interactions in a woody plant species. While the HcrVf2 gene from the wild apple Malus floribunda 821 has proved capable of conferring scab resistance to the susceptible cv. Gala after genetic transfor- mation, its identification represents only the first step in understanding the molecular mechanisms and, hence, the network of genes underlying the defence response. We used a PCR-based suppression subtractive hybridization to identify apple genes that are differentially expressed after Venturia inaequalis inoculation. Subtractive hybridization was performed between cDNA from challenged leaves of HcrVf2-resistant transgenic Gala and susceptible cv. Gala plants. A library of 523 unigenes was constructed and characterized by assigning a putative function via compar- ison with public databases. This set of pathogen-modulated apple genes includes many defence-related genes and is therefore an important source of information for under- standing the molecular basis of the Malus–V. inaequalis interaction.
|Autori:||Paris, R.;Cova, V.;Pagliarani, G.;Tartarini, S.;Komjanc, M.;Sansavini, S.|
|Data di pubblicazione:||2009|
|Titolo:||Expression profiling in HcrVf2-transformed apple plants in response to Venturia inaequalis|
|Rivista:||TREE GENETICS & GENOMES|
|Appare nelle tipologie:||1.1 Articolo in rivista|