Einkorn wheat (Triticum monococcum) is currently being studied as a cereal with reduced or null toxicity for individuals with gluten intolerance. A-PAGE and A-PAGE x SDS-PAGE eletrophoretic analysis revealed gliadin ω-331, the sole ω-gliadin in monococcum wheat ID331, in cv. Stendhal and in 25 out of 46 other accessions representative of areas of occurrence of this cereal species. ID331 and Stendhal were found to be unable to agglutinate K562(S) cells, demonstrating their reduced or null toxicity for celiac patients. By contrast, cv. Monlis, devoid of ω-gliadins, proved to be as toxic as bread wheat. Mass spectrometry of ω-331 assigned this molecule to the A family of ω-gliadins, and revealed the presence of the QQPQRPQPF nonapeptide with potential anti-inflammatory activity. The present results are discussed in relation with the development of wheat products for prevention of gluten intolerance.
|Autori:||Carrella, S.;Mazza, M.;Acutis, P.L.;D’Ambrosio, C.;Scaloni, A.;Candiano, G.;Baldassari, I.;Galassi, E.;Gazza, L.;Pogna, N.|
|Data di pubblicazione:||2013|
|Titolo:||Celiachia e grano monococco: analisi elettroforetiche e spettrometriche di ω-gliadine con potenziale attività antinfiammatoria|
|Appare nelle tipologie:||1.1 Articolo in rivista|